- Link:
- http://hdl.handle.net/1783.1/3928
- Collection:
-
- Subjects
- Chlamydomonas reinhardtii -- Genetics Chlamydomonas reinhardtii -- Effect of heavy metals on Chlamydomonas reinhardtii -- Effect of heavy metals
on
- Creator:
- Chan, Chun Tat
- Format
- 341 bytes
- Format
- text/html
- Language
- en_US
- Type
- Thesis
- Description
- Thesis (M.Phil.)--Hong Kong University of Science
and Technology, 2007
- Description
- xix, 114 leaves : ill. (some col.) ; 30
cm
- Description
- HKUST Call Number: Thesis BIOL 2007
Chan
- Description
- Heavy metals are serious environmental pollutants
due to their toxicities and bioaccumulation effects. In this study,
genes related to heavy metal responses in Chlamydomonas
reinhardtii, a unicellular green algae model species with its
entire genome sequenced, were characterized. Cadmium (Cd) is one of
the major metal pollutants. Two mutant strains, Cds18 and CdrB2
with Cd sensitive and resistance respectively, were generated by
insertational mutagensis in our laboratory previously. In mutant
Cds18, CrMRP2 coding for an ABC transporter and in CdrB2, CrPMA2
coding for a P type ATPase were altered by the insertion. I
performed a metal(loid)s resistance assay for both strains using
the wild type strain C. reinhardtii 950 as control. Cds18 was
sensitive to all the metal(loid)s studied, indicating the
multi-metals responses of CrMRP2. CdrB2 showed responses to
particular metals, in addition to Cd, the strain was resistance to
Nickel (Ni) and Zinc (Zn) among the metal(loid)s tested. Expression
of CrMRP2-GFP fusion protein in S. cerevisiae illustrated the
vacuolar localization of gene products in yeast. The transcriptions
of the two genes upon Cd, Ni and Zn stimulations were studied and
related to the metal contents incorporated into wild type, Cds18
and CdrB2 cells. In wild type cells, CrMRP2 transcription can be
induced by Cd, Ni and Zn. Wild type cells with CrMRP2 functioning
has a higher ability to detoxify intracellular metal content. While
the transcription of altered CrPMA2 is induced by Cd, Ni and Zn,
metal contents in CdrB2 declined in late part of metal exposure.
The information of this study contributed to the understanding of
cellular detoxification mechanisms for heavy metals in
photosynthetic organisms.
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